Journal: bioRxiv
Article Title: Proton sponge or membrane fusion? – Endosomal escape of siRNA polyplexes illuminated by molecular dynamics simulations
doi: 10.64898/2026.03.13.711661
Figure Lengend Snippet: A. Molecular structures of polymers used in this work; branched polyethylenimine (bPEI), poly(beta)aminoester (PBAE) with varying ratios of hydrophilic spermine and hydrophobic oleylamine (OA), and bPEI – polycaprolactone (PCL) – bPEI block copolymer (PPP). B. CG model particles with three siRNA molecules each at pH 7.4. The LNP is additionally shown with transparent lipids to visualize the orientation of the siRNA molecules inside; polymer in gold/orange, MC3/MC3H in pink, DSPC in purple, cholesterol in green, RNA in blue.
Article Snippet: Spermine and oleylamine for the synthesis of the poly(beta)aminoesters (PBAE) were obtained from Fisher Scientific (Acros, USA), whereas the 1,4-butanediol diacrylate for the PBAE backbone originated from Tokyo Chemical Industry Co. (Tokyo, Japan).
Techniques: Blocking Assay, Polymer